Walleye pollock eggs from the Gulf of Alaska were reared to the juvenile stage at 6-8°C to understand otolith increment formation and morphology. The fish were immersed in a solution of alizarin complexone (ALC) once a week for 6 hours. ALC binds readily to calcium and is laid down on the otolith during the daily increment process. Here the ALC shows up as light bands on the otolith.